Video about vgff:
Although serological methods such as ELISAs, immunofluorescence assays and neutralization tests are useful and widely applied to OPV and PPV diagnosis, these techniques cannot differentiate anti-OPV antibodies resulting from acute infection from anti-OPV antibodies resulting from a prior vaccination [ 17 ]; additionally, the titer of anti-PPV neutralizing antibodies can promptly decrease to undetectable levels a few months after the infection [ 18 ]. In the present work, we report the development of a nested-multiplex PCR system for the sensitive and reliable detection of OPV and PPV based on the combination and optimization of published primer sets. Rapid, useful and highly specific methods are required to detect and epidemiologically monitor such poxviruses.
We also report its application for the detection of viruses included in these genera directly from bovine, ovine, caprine and human exanthematic lesions with no viral isolation or DNA manipulation. Several species included in these genera are related with worldwide acute exanthematic disease in humans and domestic animals, which cause serious economic losses and impact public health [ 1 , 2 ].
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In the present work, we report the development of a nested-multiplex PCR system for the sensitive and reliable detection of OPV and PPV based on the combination and optimization of published primer sets. In the present paper, we describe the development of a nested-multiplex PCR method for the simultaneous detection of OPV and PPV species directly from exanthematic lesions, with no previous viral isolation or DNA extraction.